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Mouse VIM (Vimentin) Sandwich ELISA Kit Ready-To-Use (STJE0019430)

詳情介紹：

Applications:	ELISA
Reactivity:	Mouse
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity:	0.054ng/mL
Detection Limit:	0.156-10ng/mL
Short Description:	This VIM Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	3 hrs

Gene Symbol:	Vim
Gene ID:	22352
Uniprot ID:	VIME_MOUSE
Immunogen Region:	Ready-To-Use
Sample Type:	serum, plasma, tissue homogenates or other biological fluids.

Tissue Specificity	Detected in eye lens fiber cells (at protein level). Expressed in retinal lens epithelial cells (at protein level). Expressed in Langerhans cells in the epidermis (at protein level).
Post Translational Modifications	Phosphorylation by PKN1 inhibits the formation of filaments. Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Function	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Protein Name	Vimentin
Database Links	Reactome: R-MMU-264870 Reactome: -MMU-390522 Reactome: -MMU-9013422 Reactome: -MMU-9646399
Cellular Localisation	Cytoplasm Cytoskeleton Nucleus Matrix Cell Membrane
Alternative ELISA Names	Vimentin ELISA kit Vim ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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