Alpha actinins belong to the spectrin superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This protein is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and is thought to be involved in metastatic processes. Mutations in the gene encoding this protein have been associated with focal and segmental glomerulosclerosis.

Target	Alpha Actinin 4 (ACTN4)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC, FCM
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/10 - 1/50, FCM: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 785-813 amino acids from the C-terminal region of human ACTN4.
Isotype	IgG
Form	Liquid
Purification	Purified Rabbit Polyclonal Antibody.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	O43707 (UniProt, ExPASy)
UniProt Secondary AC	A4K467, D6PXK4, O76048
UniProt Entry Name	ACTN4_HUMAN
Gene Symbol	ACTN4
KEGG	hsa:81
String	9606.ENSP00000252699
Molecular Weight	Calculated MW: 105 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Alpha Actinin 4 (ACTN4)