There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The genes for the first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. This protein is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.

Target	Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)
Clonality	Polyclonal
Reactivity	Human, Mouse
Tested Applications	ELISA, WB, IHC, IF/ICC, FCM
Host	Rabbit
Recommended dilutions	WB: 1/2000, IHC-P: 1/500, IF/ICC: 1/10 - 1/50, FCM: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 217-246 amino acids from the Central region of human ALPL.
Isotype	IgG
Form	Liquid
Purification	Purified Rabbit Polyclonal Antibody.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P05186 (UniProt, ExPASy)
UniProt Entry Name	PPBT_HUMAN
NCBI Accession	NP_000469.3 NP_001120973.2 NP_001170991.1
KEGG	hsa:249
String	9606.ENSP00000363973
Molecular Weight	Calculated MW: 57.3 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)