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產(chǎn)品詳情

  • 產(chǎn)品名稱：Advanced Glycation End Product (AGE) Protein

  • 產(chǎn)品型號(hào)：abx655930
  • 產(chǎn)品廠商：abbexa
  • 產(chǎn)品文檔：
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簡(jiǎn)單介紹：
Advanced Glycation End Product (AGE) Protein
詳情介紹：
Advanced Glycation End Product (AGE) Protein (Active) is an active protein for use in cell culture and activity assays.

Target Advanced Glycation End Product (AGE)
Origin General
Expression Native
Tested Applications SDS-PAGE
Host Human
Conjugation Unconjugated
Form Lyophilized
Purity > 90% (SDS-PAGE)
Purification Purified by salt co-precipitation and ion-exchange chromatography.
Reconstitution To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Sequence This product is a native protein and the sequence has not been determined.
Buffer Prior to lyophilization: 10 mM PBS, pH 7.4, containing 5% Trehalose.
Activity Active
Biological Activity Glucose and other reducing sugars can react non-enzymatically with the amino groups of proteins to form compounds called advanced glycation end products (AGEs). AGEs exert their cellular functions via the interaction with receptor for advanced glycation end products (RAGE). It has been reported that AGE stimulates the differentiation and proliferation of 3T3, therefore a proliferation assay was conducted using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells in 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free DMEM prior to the addition of various concentrations of AGE. After incubation for 48 h, cells were observed by inverted microscope (Figures 1 and 2) and cell proliferation was measured by Cell Counting Kit-8 (CCK-8) assay (Figure 3). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C.
Endotoxin Level < 1.0 EU/μg protein (LAL method)
Concentration Prior to lyophilization: 2000 μg/ml
Availability Shipped within 5-15 working days.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Research Articles on Advanced Glycation End Product (AGE)
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