ABL1 is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t (9;22) translocation results in the head-to-tail fusion of the BCR and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.

Target	ABL1 (pY245)
Clonality	Polyclonal
Target Modification	Tyr245
Modification	Phosphorylation
Reactivity	Human, Mouse
Tested Applications	ELISA, WB, IHC, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100, IF/ICC: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y245 of human ABL1.
Isotype	IgG
Form	Liquid
Purification	Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P00519 (UniProt, ExPASy)
UniProt Secondary AC	A3KFJ3, Q13869, Q13870, Q16133, Q17R61, Q45F09
UniProt Entry Name	ABL1_HUMAN
Gene Symbol	ABL1
GeneID	25
OMIM	189980
HGNC	76
KEGG	hsa:25
Ensembl	ENSG00000097007
String	9606.ENSP00000361423
Molecular Weight	Calculated MW: 123 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on ABL1 (pY245)