Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Target	6-Phosphofructo-2-Kinase/fructose-2,6-Bisphosphatase 1 (PFKFB1)
Clonality	Polyclonal
Reactivity	Human, Mouse
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 13-43 amino acids from the N-terminal region of human PFKFB1.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P16118 (UniProt, ExPASy)
UniProt Secondary AC	B2RA88, B4DUN5, Q5JXS5, Q99951
UniProt Entry Name	F261_HUMAN
KEGG	hsa:5207
String	9606.ENSP00000364145
Molecular Weight	Calculated MW: 54.7 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Rat and Cow PFKFB1.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on 6-Phosphofructo-2-Kinase/fructose-2,6-Bisphosphatase 1 (PFKFB1)