The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Target	5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-2 (AMPK alpha 2)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 453-483 amino acids from the C-terminal region of human AMPK alpha2 (PRKAA2).
Isotype	IgG
Form	Liquid
Purification	Purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P54646 (UniProt, ExPASy)
UniProt Secondary AC	Q9H1E8, Q9UD43
UniProt Entry Name	AAPK2_HUMAN
KEGG	hsa:5563
String	9606.ENSP00000360290
Molecular Weight	Calculated MW: 62.3 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse and Rat PRKAA2.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-2 (AMPK alpha 2)