Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Target	5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1 (PRKAA1)
Clonality	Monoclonal
Reactivity	Human
Tested Applications	ELISA, WB
Host	Mouse
Recommended dilutions	WB: 1/500 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Human PRKAA1 recombinant protein.
Isotype	IgG1 Kappa
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q13131 (UniProt, ExPASy)
UniProt Secondary AC	A8MTQ6, B2R7E1, O00286, Q5D0E1, Q86VS1, Q9UNQ4
UniProt Entry Name	AAPK1_HUMAN
KEGG	hsa:5562
String	9606.ENSP00000346148
Molecular Weight	Calculated MW: 64 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Rat PRKAA1.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1 (PRKAA1)