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產(chǎn)品詳情

  • 產(chǎn)品名稱：26S Proteasome Complex Subunit SEM1 (SEM1) siRNA

  • 產(chǎn)品型號：abx933291
  • 產(chǎn)品廠商：abbexa
  • 產(chǎn)品文檔：
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簡單介紹：
26S Proteasome Complex Subunit SEM1 (SEM1) siRNA
詳情介紹：
siRNA to inhibit SHFM1 expression using RNA interference.

This product is provided as three 5 nmol vials (15 nmol) or 2x three 5 nmol vials (30 nmol) of lyophilized siRNA oligo duplexes. Each vial contains slightly different sequences to ensure full knockout of the gene. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot.

Target 26S Proteasome Complex Subunit SEM1 (SEM1)
Reactivity Mouse
Tested Applications RNAi
Host Synthetic
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.

Plate (wells) Final Medium Volume (ml) Final siRNA Concentration (nM) 20 μM siRNA Volume (μl) Lipofectamine 2000 Volume (μl)
960.1 1000.5 0.25
50 0.25 0.25
100.05 0.25
240.5 1002.5 1
50 1.25 1
100.25 1
121 1005 2
50 2.5 2
100.5 2
62 10010 5
50 5 5
101 5
Form Lyophilized
Purity > 97%
Quality Control Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Storage Shipped at 4 °C. Store at -20 °C for up to one year.
UniProt Primary AC P60897 (UniProt, ExPASy)
Gene Symbol SHFM1
GeneID 20422
NCBI Accession NM_009169.2
Specificity SHFM1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Availability Shipped within 7-15 working days.
Note This product is for research use only.
Directions for use
  • Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube.
  • Resuspend the siRNA oligos to an appropriate concentration with DEPC water (e.g. resuspend one vial of 5 nmol siRNA oligo in 250 μl of DEPC water for a final concentration of 20 μM).
  • Transfect with 10 nM - 100 nM siRNA 48 to 72 hours prior to cell lysis.
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